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mouse primary antibody against pdgfrα  (Novus Biologicals)


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    Novus Biologicals mouse primary antibody against pdgfrα
    Mouse Primary Antibody Against Pdgfrα, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+primary+antibody+against+pdgfr%CE%B1/PDGFR+alpha+Antibody+(8E12F2)+-+BSA+Free/pmc13099151-59-10-16
    Average 93 stars, based on 2 article reviews
    mouse primary antibody against pdgfrα - by Bioz Stars, 2026-09
    93/100 stars

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    Incubation:

    Article Title: Involvement of Fibro-Adipogenic Progenitors and Cellular Communication Network Family Signaling in the Impaired Muscle Regeneration of Wooden Breast
    Article Snippet: Sections were then incubated with blocking reagent (Blocking one Histo; Nacalai Tesque Inc.) for 10 min at room temperature. .. For PDGFRα detection, sections were incubated overnight at 4°C with mouse primary antibody against PDGFRα (8E12F2; Novus Biologicals, LLC, Littleton, CO, USA), diluted 1:2,000 (0.5 μg/mL) in immune reaction enhancer solution (Can Get Signal immunostain Solution A; TOYOBO Co., Ltd., Osaka, Japan). .. After three washes with 0.01 M phosphate-buffered saline (PBS), the sections were incubated with polymer horseradish peroxidase (HRP)-conjugated secondary antibody (Opal Multiplex IHC Kit; Perkin Elmer Co., Waltham, MA, USA) for 10 min at room temperature, and the immune complex was visualized with a fluorophore solution (Opal 520 Fluorophore; Perkin Elmer Co.) for 10 min at room temperature.



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    mouse primary antibody against pdgfrα  (Novus Biologicals)
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    Novus Biologicals mouse primary antibody against pdgfrα
    Mouse Primary Antibody Against Pdgfrα, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+primary+antibody+against+pdgfr%CE%B1/PDGFR+alpha+Antibody+(8E12F2)+-+BSA+Free/pmc13099151-59-10-16
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    primary antibody against pdgfrα  (R&D Systems)
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    R&D Systems primary antibody against pdgfrα
    FIGURE 3 Weight cycling by 1-wk HF-LF diet switch protocol induces preadipocyte proliferation and vascular remodeling, but less inflammation in eWAT. A, Representative H&E-stained eWAT (top), adipocyte size distribution (bottom left) and average adipocyte size (bottom right), of different groups. B, Representative Ki67 and <t>PDGFRα</t> <t>staining</t> (top-panel) with quantification results (bottom-panel) of different groups. Green, Ki67 stained cells; Red, PDGFRα stained cells; Blue, DAPI stained nuclei. C, Representative F4/80, CD40 and CD163 staining (top) and quantification results (bottom) of different groups. Brown, positive stained cells. D, quantitative PCR (qPCR) results of markers of M1 (top) or M2 macrophage (bottom) of different groups. E, Representative αSMA staining (left) and quantification results (right) of different groups. F, qPCR results of Vegfa, Vegfb, and Vwf of different groups. n = 3-5 per group. *P < .05 compared with LF group; #P < .05 compared with HF group; scale bar, 100 μm
    Primary Antibody Against Pdgfrα, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+primary+antibody+against+pdgfr%CE%B1/Mouse+PDGF+R+alpha+Antibody/10__1096_slash_fj__201902708r-57-3-10
    Average 96 stars, based on 1 article reviews
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    FIGURE 3 Weight cycling by 1-wk HF-LF diet switch protocol induces preadipocyte proliferation and vascular remodeling, but less inflammation in eWAT. A, Representative H&E-stained eWAT (top), adipocyte size distribution (bottom left) and average adipocyte size (bottom right), of different groups. B, Representative Ki67 and PDGFRα staining (top-panel) with quantification results (bottom-panel) of different groups. Green, Ki67 stained cells; Red, PDGFRα stained cells; Blue, DAPI stained nuclei. C, Representative F4/80, CD40 and CD163 staining (top) and quantification results (bottom) of different groups. Brown, positive stained cells. D, quantitative PCR (qPCR) results of markers of M1 (top) or M2 macrophage (bottom) of different groups. E, Representative αSMA staining (left) and quantification results (right) of different groups. F, qPCR results of Vegfa, Vegfb, and Vwf of different groups. n = 3-5 per group. *P < .05 compared with LF group; #P < .05 compared with HF group; scale bar, 100 μm

    Journal: The FASEB Journal

    Article Title: Lmo4‐resistin signaling contributes to adipose tissue‐liver crosstalk upon weight cycling

    doi: 10.1096/fj.201902708r

    Figure Lengend Snippet: FIGURE 3 Weight cycling by 1-wk HF-LF diet switch protocol induces preadipocyte proliferation and vascular remodeling, but less inflammation in eWAT. A, Representative H&E-stained eWAT (top), adipocyte size distribution (bottom left) and average adipocyte size (bottom right), of different groups. B, Representative Ki67 and PDGFRα staining (top-panel) with quantification results (bottom-panel) of different groups. Green, Ki67 stained cells; Red, PDGFRα stained cells; Blue, DAPI stained nuclei. C, Representative F4/80, CD40 and CD163 staining (top) and quantification results (bottom) of different groups. Brown, positive stained cells. D, quantitative PCR (qPCR) results of markers of M1 (top) or M2 macrophage (bottom) of different groups. E, Representative αSMA staining (left) and quantification results (right) of different groups. F, qPCR results of Vegfa, Vegfb, and Vwf of different groups. n = 3-5 per group. *P < .05 compared with LF group; #P < .05 compared with HF group; scale bar, 100 μm

    Article Snippet: For immunofluorescence staining, primary antibody against PDGFRα (AF1062, 1:200 dilution, R&D Systems) or Ki67 (sc15402, 1:100 dilution, Santa Cruz) was applied overnight.

    Techniques: Staining, Real-time Polymerase Chain Reaction